Enhancing Aloe Vera growth and secondary metabolite production using an alternating magnetic field

Document Type : Short Communication

Authors

1 Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran

2 Department of Agriculture, Islamic Azad University, Damghan, Iran

Abstract

Magnetic field can be used as a physical elicitor to increase secondary metabolite in medicinal plants. In this research, the effects of alternating magnetic stress on production of flavonoids and growth parameters of Aloe vera were studied. Number of weeks, magnetic flux density, and exposure time are effective parameters that have been changed in this experiment. In order to apply alternating magnetic field, a system was designed and built, including Helm Holtz coil, auto-transformer, multi meter, and Tesla meter. Control samples were grown without magnetic field.  All samples were kept in growth chamber at a temperature of 24 ± 2° C and 16-hour light, 8-hour dark photoperiod. Factorial experiment based on RCD used to test magnetic field effects on selected traits. According to the obtained results, the magnetic field was effective on secondary metabolite production. 38.44 mg/g DW flavonoid was produced by with flux density of 1.5 mT and 45 min per day exposure times for two weeks. The maximum number of leaves was observed in 0.5 mT and 135 min per day exposure times. In addition, the highest plant was produced after three weeks with 0.5 mT. The week, exposure time and the magnetic flux density have second order interaction. It seems that the magnetic field can be both harmful and beneficial for Aloe vera that is dependent on various factors. In order to achieve the desired result, optimum field should be used.

Keywords

Main Subjects


Abdani Nasiri, A., Mortazaeinezhad, F., and Taheri, R. (2018). Seed germination of medicinal sage is affected by gibberellic acid, magnetic field and laser irradiation Electromagnetic Biology and Medicine, 37(1), 50-56. https://doi.org/10.1080/15368378.2017.1336100.
Baghel, L., Kataria, S., and Jain, M. (2019). Mitigation of adverse effects of salt stress on germination, growth, photosynthetic effciency and yield in maize (Zea mays L.) through magnetopriming. Acta Agrobotanica, 72, 1-16. DOI:10.5586/aa.1757
Bezerra, E. A., Carvalho, C. P. S., Filho, R. N. C., and Alam, A. F. B. S. M. (2023). Static magnetic field promotes faster germination and increases germination rate of Calotropis procera seeds stimulating cellular metabolism. Biocatalysis and Agricultural Biotechnology, 49, 24-29.
Bukhari, S. A., Tanveer, M., Mustafa, G., and Zia- Ud-Den, N. (2021). Magnetic field stimulation effect on germination and antioxidant activities of presown hybrid seeds of sunflower and its seedlings. Hindawi Journal of Food Quality, 2021, 1-9. https://doi.org/ 10.1155/2021/6693587
Deamici, K. M., Cardias, B. B., Costa, J. A. V., and Santos, L. O. (2016). Static magnetic fields in culture of Chlorella fusca: Bioeffects on growth and biomass composition. Process Biochemistry, 51, 912-912. https://doi.org/ 10.1016/j.procbio.2016.03.010
Dhawi, F., and Al-Khayri, J. M. (2009). Magnetic fields induce changes in photosynthetic pigments content in date palm (Phoenix dactylifera L.) seedlings. The open agriculture journal, 3, 1-5. https://doi.org/ 10.2174/1874331500903010001
Ercan, I., Tombuloglu, H., Alqahtani, N., Alotaibi, B., Bamhrez, M., and Alshumrani, R. (2022). Magnetic field effects on the magnetic properties, germination, chlorophyll fluorescence, and nutrient content of barley (Hordeum vulgare L.). Plant Physiology and Biochemistry, 170, 36-48. https://doi.org/ 10.1016/j.plaphy.2022.02.010
Florez, M., Alvarez, J., Martinez, E., and Carbonell, V. (2019). Stationary magnetic field stimulates rice roots growth. Romanian Reports in Physics, 71(713-718). https://doi.org/ 10.3139/145.110.010
Hajnorouzi, A., Vaezzadeh, M., Ghanati, F., and Nahidian, B. (2011). Growth promotion and a decrease of oxidative stress in maize seedlings by a combination of geomagnetic and weak electromagnetic fields. Journal of Plant Physiology, 168, 1123-1128. https://doi.org/ 10.1016/j.jplph.2010.11.010
Kataria, S., Baghel, L., Jain, M., and Guruprasad, K. (2019). Magnetopriming regulates antioxidant defense system in soybean against salt stress. Biocatalysis and Agricultural Biotechnology, 18, 101-109. https://doi.org/ 10.1016/j.bcab.2019.101097
Kaur, S., Vian, A., Chandel, S., Batish, D. R., and Kohli, R. K. (2021). Sensitivity of plants to high frequency electromagnetic radiation: cellular mechanisms and morphological changes. Reviews in Environmental Science and Biotechnology, 20(1), 55-74. https://doi.org/ 10.1007/s11157-020-09507-0
Khan, M. S., Yusufzai, S. K., Rafatullah, M., Sarjadi, M. S., and Razlan, M. (2018). Determination of total phenolic content, total flavonoid content and antioxidant activity of vVarious organic crude extracts of Licuala Spinosa leaves from Sabah, Malaysia ASM Science Journal, 11(3), 53-58. https://doi.org/ 10.21315/asmsj2018.11.3.6
Martinez, E., Florez, M., and Carbonell, M. (2017). Stimulatory effect of the magnetic treatment on the germination of cereal seeds. International Journal of Agriculture Environment and Biotechnology, 2, 375-381. https://doi.org/ 10.5958/2230-732X.201
Menegatti, R. D., Oliveira, L. O. d., Costa, Á. V. L. d., Jacira, E., Braga, B., and Bianch, V. J. (2019). Magnetic field and gibberelic acid as pre-germination treatments of passion fruit seeds. Agricultural Science Magazine, 17, 1522. https://doi.org/10.28998/rca.v17i1.6522
Mghaiouini, R., Elaouad, A., Taimoury, H., Sabir, I., Chibi, F., Hozayn, M., Garmim, T., Nmila, R., Rchid, H., and Monkade, M. (2020). Influence of the electromagnetic device aqua 4D on water quality and germination of lettuce (Lactuca sativa L.). International Journal of Current Engineering and Technology, 10(1), 19-24. https://doi.org/10.14741/ijcet/v.10.1.4
Mohammadi, F., Ghanati, F., Sharifi, M., and Chashmi, N. A. (2018). On the mechanism of the cell cycle control of suspension-cultured tobacco cells after exposure to static magnetic field. Plant Science, 277, 139–144. https://doi.org/10.1016/j.plantsci.2018.08.004
Omid, S. (2016). The effect of magnetized water on physiological and agronomic traits of cowpea (Vigna unguiculata L.). Journal of Research in Chemical, Metallurgical and Civil Engineer, 26, 195-197. DOI:10.15242/IJRCMCE.IAE0716403
Racuciu, M., Iftode, C., and Miclaus, S. (2015). Inhibitory effects of low thermal radiofrequency radiation on physiological parameters of Zea mays seedlings growth. Romanian Journal of Physics, 60(3), 603-612. https://doi.org/ 10.15242/ijrcmce.iae0716403
Radhakrishnan, R., Leelapriya, T., and Kumari, B. D. R. (2012). Effects of pulsed magnetic field treatment of soybean seeds on calli growth, cell damage, and biochemical changes under salt stress. Bioelectromagnetics, 33, 670-681. https://doi.org/10.1002/bem.21759
Sarraf, M., Kataria, S., Taimourya, H., Santos, L. O., Menegatti, R. D., Jain, M., Ihtisham, M., and Liu, S. (2020). Magnetic Field (MF) Applications in Plants: An Overview. Plants, 1139(9), 1-17. https://doi.org/ 10.3390/plants9091139
Sunita, K., Lokesh, B. K. N., and Guruprasad. (2017). Pre-treatment of seeds with static magnetic field improves germination and early growth characteristics under salt stress in maize and soybean. Journal of Biocatalysis and Agricultural Biotechnology, 10, 83-90. https://doi.org/ 10.1016/j.bcab.2017.02.010
Taimourya, H., Oussible, M., Baamal, L., Harif, A., Zaid, E., Guedira, A., and Smouni, A. (2017). Magnetic Treatment of Culture Medium Enhance Growth and Minerals Uptake of Strawberry (Fragaria ananassa Duch.) and Tomato (Solanum lycopersicum) in Fe Deficiency Conditions. International Journal of Scientific Engineering and Research, 8, 1414-1436.
Vian, A., Davies, E., Gendraud, M., and Bonnet, P. (2016). Plant responses to high frequency electromagnetic fields. BioMed Research International, 201(1-13). https://doi.org/10.1155/2016/2987192
Xia, X., Pagano, A., Macovei, A., Padula, G., Balestrazzi, A., and Hołubowicz, R. (2024). Magnetic field treatment on horticultural and agricultural crops: its benefits and challenges. Folia Horticulture, 36(1), 67-80. https://doi.org/10.2478/fhort-2024-0004
Xu, Y., Zhang, R., and Fu, H. (2005). Studies on the optimal process to extract flavonoids from Red-raspberry fruits. Nature and Science, 3(3), 43-46.