Isolation and Screening of Antibacterial-Producing Endophytic Actinomycetes from Hollyhock (Alcea rosea)

Document Type : Research Paper

Authors

Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran

Abstract

Actinomycete bacteria are widely recognized for their ability to produce a diverse array of bioactive compounds, particularly antibiotics, and contribute significantly to the discovery of naturally derived antimicrobial agents. This study focused on the isolation and characterization of actinomycete strains from hollyhock (Alcea rosea) plant tissues to assess their antibacterial potential against clinically relevant pathogens. The target pathogens included Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. A total of 110 bacterial isolates were obtained, with 62 isolates confirmed as Actinomycetes through PCR amplification of the 16S rRNA gene. Approximately 46.5% of these isolates exhibited antibacterial activity, with pronounced effects against B. cereus and S. aureus, suggesting their potential role in combating antibiotic-resistant bacteria. Genetic analysis revealed the presence of non-ribosomal peptide synthetase (NRPS) genes, indicating that the produced antimicrobial compounds may originate from NRPS pathways. However, no polyketide synthase (PKS) genes were detected, which may limit the diversity of bioactive metabolites. These findings highlight the pharmaceutical potential of hollyhock-associated Actinomycetes as promising sources of novel antimicrobial agents.

Keywords

Main Subjects

References

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Microbiology, Metabolites and Biotechnology
Volume 7, Issue 2
December 2024
Pages 92-101

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