Evaluation of the potential of two native microalgae isolates of the Persian Gulf in different culture scales for possible bioethanol production

Document Type : Research Paper

Authors

1 Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

2 Department of Biotechnology Iranian Reasearch Organization for Science and Technology, Tehran, P. O. Box 3353-5111, Iran.

Abstract

In the wake of extensive fossil fuel use and CO2 accumulation in the environment, biofuel production from microalgae may be more effective and leave a less environmental footprint. The nutritional and environmental factors and their interactions affect the growth performance and biochemical constitution of different microalgae. The behavior of microalgal cells in different culture scales depends on the mentioned factors. The present study evaluates the potential of two microalgae isolates, Picochlorum D8, and Chlorella S4, in different culture scales. High biomass and carbohydrate productivity were considered as important factors to identify the potent microalga. Acid-thermal pretreatment was applied to measure the carbohydrate concentration. The carbohydrate composition of selected microalga was investigated using thin-layer chromatography (TLC). According to the observations, Chlorella S4 exhibited the best dry biomass and carbohydrate productivity of 62 ± 6 mg/L/d and 19.16 ± 1.57 mg/L/d in a 200 L indoor open raceway pond, respectively. For Picochlorum D8, the highest biomass productivity of 26.24 ± 0.625 mg/L/d and carbohydrate productivity of 7.45 ± 0.53 mg/L/d were achieved in a 2 L Erlenmeyer flask. Based on TLC analysis, glucose, galactose, and xylose were detected as the main monosaccharides in Chlorella S4 hydrolysate. The current study demonstrated Chlorella S4’s capacity to produce biomass in a large-scale system. The relatively high carbohydrate content of this microalga makes it a promising raw material for potentially producing bioethanol.

Keywords

References

 
[1] Alexander, S.M., Retnakumar, R.J., Chouhan, D., Devi, T.N.B., Dharmaseelan, S., Devadas, K., Thapa, N., Tamang, J.P., Lamtha, S.C. and Chattopadhyay, S. (2021) Helicobacter pylori in Human Stomach: The Inconsistencies in Clinical Outcomes and the Probable Causes. Front Microbiol 12:713955. doi: 10.3389/fmicb.2021.713955.
[2] Amabebe, E. and Anumba, DOC. (2020) Female Gut and Genital Tract Microbiota-Induced Crosstalk and Differential Effects of Short-Chain Fatty Acids on Immune Sequelae. Front Immunol 11:2184. doi: 10.3389/fimmu.2020.02184.
[3] Bravo, D., Hoare, A., Soto, C., Valenzuela, M.A. and Quest, A.F. (2018) Helicobacter pylori in human health and disease: Mechanisms for local gastric and systemic effects. World J Gastroenterol 24(28):3071-3089. doi: 10.3748/wjg. v24.i28.3071.
[4] Buggs, C. and Rosenfield, R.L. (2005) Polycystic ovary syndrome in adolescence. Endocrinol Metab Clin North Am 34(3):677-705, x. doi: 10.1016/j.ecl.2005.04.005.
[5] Campuzano-Maya, G. (2014) Hematologic manifestations of Helicobacter pylori infection. World J Gastroenterol 20(36):12818-38. doi: 10.3748/wjg.v20.i36.12818.
[6] Chen, X., Wang, N., Wang, J., Liao, B., Cheng, L. and Ren, B. (2022) The interactions between oral-gut axis microbiota and Helicobacter pylori. Front. Cell. Infect. Microbiol 12:914418. doi: 10.3389/fcimb.2022.914418
[7] Darvishi, M., Forootan, M., Azmodeh, O., Forootan, M. and Matinfar, H. (2017) Studying the Frequency of Helicobacter Pylori Infection Among Women Suffering from Polycystic ovary Resorting to Hospital Infertility Center. Biomed Pharmacol J 10(1).
[8] Dennett, C.C. and Simon, J. (2015) The role of polycystic ovary syndrome in reproductive and metabolic health: overview and approaches for treatment. Diabetes Spectr 28(2):116-20. doi: 10.2337/diaspect.28.2.116.
[9] Deswal, R., Narwal, V., Dang, A. and Pundir, C.S. (2020) The Prevalence of Polycystic Ovary Syndrome: A Brief Systematic Review. J Hum Reprod Sci 13(4):261-271. doi: 10.4103/jhrs.JHRS_95_18.
[10] Dimitriadi, D. (2014) Helicobacter pylori: a sexually transmitted bacterium? Cent European J Urol 67(4):407-9. doi: 10.5173/ceju.2014.04. art18.
[11] El Hayek, S., Bitar, L., Hamdar, L.H., Mirza, F.G. and Daoud, G. (2016) Poly Cystic Ovarian Syndrome: An Updated Overview. Front. Physiol. 7:124. doi: 10.3389/fphys.2016.00124
[12] Eslick, G.D. (2000) Helicobacter pylori infection transmitted sexually via oral-genital contact: a hypothetical model. Sex Transm Infect 76(6):489-92. doi: 10.1136/sti.76.6.489.
 
 
[13] Faghri, J., Poursina, F., Moghim, S., Zarkesh Esfahani, H., Nasr Esfahani B., et al. (2014) Morphological and Bactericidal Effects of Different Antibiotics on Helicobacter pylori. Jundishapur J Microbiol 7(1):e8704.
[14] Farhadi-Azar, M., Behboudi-Gandevani, S., Rahmati, M., Mahboobifard F., Khalili Pouya E., Ramezani Tehrani F. and Azizi F. (2022) The Prevalence of Polycystic Ovary Syndrome, Its Phenotypes and Cardio-Metabolic Features in a Community Sample of Iranian Population: Tehran Lipid and Glucose Study. Front. Endocrinol 13:825528. doi: 10.3389/fendo.2022.825528
[15] Figura, N., Piomboni, P., Ponzetto, A., Gambera, L., Lenzi, C., Vaira, D., et al. (2002) Helicobacter pylori infection and infertility. Eur J Gastroenterol Hepatol 14: 663-9.
[16] Foroozanfard, F., Moosavi, S.G., Mansouri, F. and Bazarganipour, F. (2014) Obstetric and Neonatal Outcome in PCOS with Gestational Diabetes Mellitus. J Family Reprod Health 8(1):7-12.
[17] Hernandez, C.D., Shin, H., Troncoso, P.A., Vera, M.H., Villagran, A.A., Rodriguez-Rivera, S.M., Ortiz, M.A., Serrano, C.A., Borzutzky, A., Dominguez-Bello, M.G. and Harris, P.R. (2020) Maternal H. pylori is associated with differential fecal microbiota in infants born by vaginal delivery. Sci Rep 10(1):7305. doi: 10.1038/s41598-020-64296-7.
[18] Jabeen, A., Yamini, V., Rahman, Amberina, A., Dinesh Eshwar, M., Vadakedath, S., Begum, G.S. and Kandi, V. (2022) polycystic ovarian syndrome: Prevalence, Predisposing Factors, and Awareness Among Adolescent and Young Girls of South India. Cureus 14(8):e27943. doi: 10.7759/cureus.27943.
[19] Kazemi, E. and Khazaei, M. (2018). 'A review of the effects of Helicobacter pylori infection on reproduction, pregnancy and gynecologic diseases', The Iranian Journal of Obstetrics, Gynecology and Infertility, 21(Supplement), 67-75. doi: 10.22038/ijogi.2018.11622
[20] Khalili, Sharifi yazdi, Sadeh. (2007) Correlation of H. pylori infection and infertility; a survey in Yazd infertility clinic.Tehran University Medical Journal; Vol. 65, No. 3, Jun 2007: 72-77.
[21] Kiani, A.H., Asadbeik, E., Bibalan, M.H., Sedighi, M., Eshaghi, M., Gholami, M. and Pournajaf, A. (2015) Serological diagnosis of Helicobacter pylori infection in patients with a polycystic ovary syndrome. Archives of Clinical Infectious Diseases, 10 (2).
[22] Krzyżek, P. and Gościniak, G. (2018) Morphology of Helicobacter pylori as a result of peptidoglycan and cytoskeleton rearrangements. Prz Gastroenterol 13(3):182-195. doi: 10.5114/pg.2018.78284.
[23] Kshetrimayum, C., Sharma, A., Mishra, V.V. and Kumar, S. (2019) Polycystic ovarian syndrome: Environmental/occupational, lifestyle factors; an overview. J Turk Ger Gynecol Assoc 20(4):255-263. doi: 10.4274/jtgga.galenos.2019.2018.0142.
[24] Li, J., Fan, M., Ma, F., Zhang, S. and Li, Q. (2021) The effects of Helicobacter pylori infection on pregnancy-
related diseases and fetal development in diabetes in pregnancy. Ann Transl Med 9(8):686. doi: 10.21037/atm-21-1209.
[25] Manoharan, V. and Wong, VW. (2020) Impact of comorbid polycystic ovarian syndrome and gestational diabetes mellitus on pregnancy outcomes: a retrospective cohort study. BMC Pregnancy Childbirth 20(1):484. doi: 10.1186/s12884-020-03175-5.
[26] Melo, A.S., Ferriani, R.A. and Navarro P.A. (2015) Treatment of infertility in women with polycystic ovary syndrome: approach to clinical practice. Clinics (Sao Paulo) 70(11):765-9. doi: 10.6061/clinics/2015(11)09.
[27] Moretti, E., Figura, N., Collodel, G. and Ponzetto, A. (2014) Can Helicobacter pylori infection influence human reproduction? World J Gastroenterol 20(19):5567-74. doi: 10.3748/wjg.v20.i19.5567.
[28] Ndefo, U.A., Eaton, A. and Green, MR. (2013) Polycystic ovary syndrome: a review of treatment options with a focus on pharmacological approaches. PT 38(6):336-55.
[29] Niknam, R., Fattahi, M.R., Sepehrimanesh, M. and Safarpour, A. (2018) Prevalence of Helicobacter pylori in Southern Part of Iran. Jundishapur J Microbiol 11(6): e62379.
[30] Pakniat H., Kazemi F., Movahed F., Haji Seyed Abotorabi, SH.S., Soufizadeh, N. and Yaghobi, F. (2022) Evaluation of Pregnancy consequences in Women with Polycystic Ovary Syndrome: A Case-Control Study in Qazvin, Iran. SJKU 27(1):74-83.
[31] Pattnaik, L., Naaz, S.A., Das, B., Dash, P., Pattanaik, M. (2022) Adverse Pregnancy Outcome in Polycystic Ovarian Syndrome: A Comparative Study. Cureus 14(6): e25790. doi: 10.7759/cureus.25790.
[32] Ranathunga, I., Athukorala, T.G., Sumanatilleke, M.R. et al. (2022) Evaluation of socio-demographic and clinical characteristics of PCOS patients attending a tertiary care institute in Colombo. BMC Endocr Disord 22, 289.
[33] Rojas, J., Chávez, M., Olivar, L., Rojas, M., Morillo, J., Mejías, J., Calvo, M. and Bermúdez, V. (2014) Polycystic ovary syndrome, insulin resistance, and obesity: navigating the pathophysiologic labyrinth. Int J Reprod Med 719050. doi: 10.1155/2014/719050.
[34] Roussos, A., Philippou, N. and Gourgoulianis, K.I. (2003) Helicobacter pylori infection and respiratory diseases: a review. World J Gastroenterol 9(1):5-8. doi: 10.3748/wjg.v9.i1.5.
[35] Salih, B.A. (2009) Helicobacter pylori infection in developing countries: the burden for how long? Saudi J Gastroenterol 15(3):201-7. doi: 10.4103/1319-3767.54743.
[36] Sánchez-Alonzo, K., Matamala-Valdés, L., Parra-Sepúlveda, C., Bernasconi, H., Campos, V.L., Smith, C.T., Sáez, K. and García-Cancino, A. (2021) Intracellular Presence of Helicobacter pylori and Its Virulence-Associated Genotypes within the Vaginal Yeast of Term Pregnant Women. Microorganisms 9(1):131. doi: 10.3390/microorganisms9010131.
[37] Sawant, S. and Bhide, P. (2023) Fertility Treatment Options for Women with Polycystic Ovary Syndrome. Clin Med Insights Reprod Health. 2019. doi: 10.1177/1179558119890867.
[38] Singh, S, Pal, N, Shubham, S, Sarma, DK, Verma, V, Marotta, F, Kumar, M. (2023) Polycystic Ovary Syndrome: Etiology, Current Management, and Future Therapeutics. Journal of Clinical Medicine 12(4):1454.
[39] Sohrabvand, F., Shariat, M., Farahvash, M.J., Haghollahi, F., Khosravi, M., Maasomi, M., et al. (2014) Serological study of Helicobacter pylori infection in patients with Polycystic Ovary Syndrome. Tehran Univ Med J 71 (10) :660-664
[40] Torabi, R, Tebyanian, H, Heiat, M. and Choopani, A. (2022) Seroepidemiological Prevalence of Helicobacter pylori in the south of Tehran, Iran. Novelty in Clinical Medicine 1(1): 55-58. doi: 10.22034/ncm.2022.140810
[41] Vinette, K.M., Gibney, K.M., Proujansky, R. et al. (2002) Growth of Helicobacter pylori in a long spiral form does not alter expression of immunodominant proteins. BMC Microbiol 2, 24.
[42] Witchel, S.F., Oberfield, S.E., and Peña A.S. (2019) Polycystic Ovary Syndrome: Pathophysiology, Presentation, and Treatment with Emphasis on Adolescent Girls. J Endocr Soc 3(8):1545-1573. doi: 10.1210/js.2019-00078.
[43] Xia, B., Wang, W., Lu, Y. and Chen, C. (2020) Helicobacter pylori infection increases the risk of metabolic syndrome in pregnancy: a cohort study. Ann Transl Med 8(14):875. doi: 10.21037/atm-20-4863.
[44] Yang, S.W., Yoon, S.H., Kim, M., Seo, Y.S. and Yuk, J.S. (2023) Risk of Gestational Diabetes and Pregnancy-Induced Hypertension with a History of Polycystic Ovary Syndrome: A Nationwide Population-Based Cohort Study. Journal of Clinical Medicine 12(5):1738.
[45] Yavasoglu, I., Kucuk, M., Cildag, B., Arslan, E., Gok, M., and Kafkas, S. (2009) A novel association between polycystic ovary syndrome and Helicobacter pylori. Am J Med Sci 338(3):174–177. doi: 10.1097/MAJ.0b013e3181a63c8a
[46] Yavaşoglu, I. and Küçük, M. (2012) Anti-Helicobacter pylori antibodies and polycystic ovary syndrome. Eur J Obstet Gynecol Reprod Biol
[47] Yee, J.K.C. (2017) Are the view of Helicobacter pylori colonized in the oral cavity an illusion? Exp Mol Med 49(11): e397. doi: 10.1038/emm.2017.225.
[48] Zargham, R., Moteshakker-Arani, M. and Soleimani-Meigooni, S. (2015) Prevalence of Helicobacter pylori infection on polycystic ovary syndrome in the women visiting Shariati hospital in 2013. NPWJM 3 (6) :61-65.
[49] Zhang, L., Chen, X., Ren, B., Zhou, X. and Cheng, L. (2022) Helicobacter pylori in the Oral Cavity: Current Evidence and Potential Survival Strategies. Int. J. Mol. Sci 23, 13646.
 
Microbiology, Metabolites and Biotechnology
Volume 5, Issue 2
November 2022
Pages 72-82

Files

Share

How to cite

Statistics