Iranian Research Organization for Science and Technology (IROST) Microbiology, Metabolites and Biotechnology 2980-8855 8 2 2025 11 01 Anticoagulant Activity of Exopolysaccharide Extracted from Two Native Microalgae 73 80 1609 10.22104/mmb.2025.7805.1182 EN Zahra Mousavian Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P. O. Box 3353-5111, Tehran, Iran Maliheh Safavi Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P. O. Box 3353-5111, Tehran, Iran Farzaneh Azizmohseni Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P. O. Box 3353-5111, Tehran, Iran Saeed Mirdamadi Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P. O. Box 3353-5111, Tehran, Iran Journal Article 2025 08 05 The increasing demand for natural substances in medicinal applications has made microalgae an attractive bioresource. The potential health benefits of polysaccharides from a variety of microalgae species have garnered attention. The aim of this study was to investigate and characterize the growth, biomass, and exopolysaccharide production profile of two microalgal strains, Chlorella sp. and Chlorella sorokiniana. The anticoagulant activities of extracted sulfated polysaccharides were evaluated using activated partial thromboplastin time (aPTT) and prothrombin time (PT) assays. Higher carbohydrate production yield (19 (w/w)) was observed in Chlorella sorokiniana. Therefore, Chlorella sorokiniana can be used to produce biomass that is high in carbohydrates. The sulfated polysaccharide extracted from Chlorella sorokiniana and Chlorella sp. showed anticoagulant properties. C. sorokiniana and Chlorella sp. (strain D1) sPS were found to be less active than heparin in the measurement of aPTT and PT. To possess the same effect as heparin, greater polysaccharide concentrations were necessary These results demonstrate the potential of sulfated polysaccharides derived from microalgae to be used in the development of new therapeutic agents. The development of next-generation antithrombotic medications, which will have fewer side effects and a greater range of uses, may be facilitated by such studies.These results demonstrate the potential of sulfated polysaccharides derived from microalgae to be used in the development of new therapeutic agents. The development of next-generation antithrombotic medications, which will have fewer side effects and a greater range of uses, may be facilitated by such studies. Keywords: Microalgae, cultivation, exopolysaccharides, food supplement

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